Gating strategy for the identification of cell subpopulations in the human lung tissue model

Grau-Expósito, Judith; Perea, David; Suppi, Marina; Massana, Nuria; Vergara, Ander; Soler, María José; Trinité, Benjamin; Blanco, Julià; García-Pérez, Javier; Alcamí, José; Serrano-Mollar, Anna; Rosado, Joel; Falcó, Vicenç; Genesca, Meritxell; Buzón, María José

Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/329705

COMPARTIR / EXPORTAR:

SHARE BASE	Comparte tu historia de Acceso Abierto
Visualizar otros formatos: MARC \| Dublin Core \| RDF \| ORE \| MODS \| METS \| DIDL \| DATACITE
Refman EndNote Bibtex RefWorks Excel CSV PDF DataCite Send via email

Título:	Gating strategy for the identification of cell subpopulations in the human lung tissue model
Autor:	Grau-Expósito, Judith; Perea, David; Suppi, Marina; Massana, Nuria; Vergara, Ander; Soler, María José; Trinité, Benjamin; Blanco, Julià; García-Pérez, Javier; Alcamí, José CSIC ORCID; Serrano-Mollar, Anna CSIC ORCID; Rosado, Joel; Falcó, Vicenç; Genesca, Meritxell; Buzón, María José
Palabras clave:	term cell culture structural cell populations preserves main immune susceptible cell targets local inflammatory responses Viral entry inhibitors viral entry factors primary human cells highly reproducible method div >< p alveolar type ii new compounds missed modulate ace2 expression viral targets inflammatory compounds Viral infection new therapeutics 2 entry different compounds without long vitro rapid platform rapid method proteins involved potential therapeutics physiological models pathogen interactions identify host hlt cells hlt ), direct use differentiation approaches conventional systems antiviral testing antiviral activity also show
Fecha de publicación:	13-ene-2022
Editor:	Figshare
Citación:	Grau-Expósito, Judith; Perea, David; Suppi, Marina; Massana, Nuria; Vergara, Ander; Soler, María José; Trinité, Benjamin; Blanco, Julià; García-Pérez, Javier; Alcamí, José; Serrano-Mollar, Anna; Rosado, Joel; Falcó, Vicenç; Genesca, Meritxell; Buzón, María José; 2022; Gating strategy for the identification of cell subpopulations in the human lung tissue model [Dataset]; Figshare; https://doi.org/10.1371/journal.ppat.1010171.s002
Descripción:	(A) General gating strategy used to identify different cell subsets in lung samples. A gate based on FSC vs. SSC was followed by doublet and dead cells exclusion. From live CD45- cells, endothelial cells (CD31+, purple) and epithelial cells (EpCAM+, grey) were gated, and within epithelial cells, AT-II cells (EpCAM+ and HLA-DR+, pink) were identified. Out of live CD45+ cells and based on FSC vs. SSC, we identified a lymphocyte population in which we distinguished between non-T lymphocytes (turquoise) and T cells (dark green) based on CD3 expression; and big cells, where we identified three major subsets based on their expression of CD11b and CD11c and, subsequently, CD14 and HLA-DR markers. We identified alveolar macrophages (blue), monocytes (violet), myeloid dendritic cells (mDCs, fuchsia) and neutrophils (orange).
Versión del editor:	https://doi.org/10.1371/journal.ppat.1010171.s002
URI:	http://hdl.handle.net/10261/329705
DOI:	10.1371/journal.ppat.1010171.s002
Referencias:	Grau-Expósito, Judith; Perea, David; Suppi, Marina; Massana, Nuria; Vergara, Ander; Soler, María José; Trinité, Benjamin; Blanco, Julià; García-Pérez, Javier; Alcamí, José; Serrano-Mollar, Anna; Rosado, Joel; Falcó, Vicenç; Genesca, Meritxell; Buzón, María José. Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells. https://doi.org/10.1371/journal.ppat.1010171. http://hdl.handle.net/10261/268646
Aparece en las colecciones:	(IIBB) Conjuntos de datos (PTI Salud Global) Colección Especial COVID-19