Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/310942
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Dataset of A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability |
Autor: | Cañas, Juan C. CSIC ORCID; García-Rubio, María L. CSIC ORCID; García, Alicia CSIC ORCID; Antequera, Francisco CSIC ORCID; Gómez-González, Belén CSIC ORCID; Aguilera, Andrés CSIC ORCID | Fecha de publicación: | 13-jul-2022 | Editor: | GEO - Gene Expression Omnibus | Citación: | Cañas, Juan C.; García-Rubio, María L.; García, Alicia; Antequera, Francisco; Gómez-González, Belén; Aguilera, Andrés; 2022; Dataset of A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability [Dataset]; Gene Expression Omnibus; GSE192701 | Resumen: | The stability of the genome is occasionally challenged by the formation of DNA-RNA hybrids and R-loops, which can be influenced by the chromatin context. This is mainly due to the fact that DNA-RNA hybrids hamper the progression of replication forks, leading to fork stalling and, ultimately, DNA breaks. Through a specific screening of chromatin modifiers performed in the yeast Saccharomyces cerevisiae, we have found that the Rtt109 histone acetyltransferase is involved in several steps of R-loop-metabolism and their associated genetic instability. On one hand, Rtt109 prevents DNA-RNA hybridization by the acetylation of histone H3 lysines 14 and 23, and on the other hand, it is involved in the repair of replication-born DNA breaks, such as those that can be caused by R-loops, by acetylating lysines 14 and 56. In addition, Rtt109 loss renders cells highly sensitive to replication stress in combination with R-loop-accumulating THO-complex mutants. Our data evidence that the chromatin context simultaneously influences the occurrence of DNA-RNA hybrid-associated DNA damage and its repair, adding complexity to the source of R-loop-associated genetic instability. | Descripción: | Mononucleosomal DNA was isolated after MNase treatment and was then prepared for sequencing..-- Organism: Saccharomyces cerevisiae Resources available on the publisher's site: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE192701 |
Versión del editor: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE192701 | URI: | http://hdl.handle.net/10261/310942 | Referencias: | Cañas, Juan C.; García-Rubio, María L.; García, Alicia; Antequera, Francisco; Gómez-González, Belén; Aguilera, Andrés; 2022; A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability; Genetics; http://dx.doi.org/10.1093/genetics/iyac108; http://hdl.handle.net/10261/287529 |
Aparece en las colecciones: | (IBFG) Conjuntos de datos (CABIMER) Conjuntos de datos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
README_Saccharomyces.txt | 3,12 kB | Text | Visualizar/Abrir | |
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.