Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/296429
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Sot, Jesús | es_ES |
dc.contributor.author | Gartzia-Rivero, Leire | es_ES |
dc.contributor.author | Bañuelos, Jorge | es_ES |
dc.contributor.author | Goñi, Félix M. | es_ES |
dc.contributor.author | Alonso, Alicia | es_ES |
dc.date.accessioned | 2023-03-02T10:12:50Z | - |
dc.date.available | 2023-03-02T10:12:50Z | - |
dc.date.issued | 2022-10-01 | - |
dc.identifier.citation | Journal of Molecular Liquids 363: 119874 (2022) | es_ES |
dc.identifier.issn | 0167-7322 | - |
dc.identifier.uri | http://hdl.handle.net/10261/296429 | - |
dc.description.abstract | The basic matrix of cellular membranes consists of a double layer (bilayer) of phospholipids. Semisynthetic lipid bilayers are commonly used in biophysical studies of membranes. According to temperature and composition, lipid bilayers can exist in liquid-crystalline (or liquid-disordered), liquid-ordered, rippled, and gel phases. In the present study, the hydrophobic, solvatochromic molecule Nile red has been used as a fluorescent probe to examine the physical state of bilayers of different compositions in the 15–60 °C range. Phospholipids with saturated or unsaturated acyl chains, in the presence or absence of cholesterol have been studied. Nile red shows absorption maxima at 520–550 nm and emission maxima at 580–640 nm, single photon excitation not being damaging to the system. A red/orange intensity ratio (ROIR) index has been used to normalize the results. ROIR varies clearly and reproducibly with the lipid phase, increasing in the order: liquid-ordered < gel < rippled < liquid-crystalline. It increases with temperature and decreases with cholesterol contents in the bilayers. Nile red allows an unusually clear observation of the rippled-to-liquid crystalline phase transition in saturated phospholipids. FLIM studies with Nile red also show differences between lamellar phases. Rotational relaxation times have been determined for Nile red in liquid-disordered (0.72 ± 0.010 ns), gel (1.16 ± 0.070 ns), and liquid-ordered (1.79 ± 0.14 ns) phases, the large value of the liquid-ordered phase being an indication of the sterol hindering probe tumbling in the hydrophobic matrix. | es_ES |
dc.description.sponsorship | This work was supported in part by the Spanish Ministerio de Ciencia e Innovación (MCI), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (grants No. PGC2018-099857-B-I00, PID2020-114755GB-C33 ), by the Basque Government (grants No. IT1625-22, IT1639-22,and IT1270-19), by Fundación Ramón Areces (CIVP20A6619), by Fundación Biofísica Bizkaia, and by the Basque Excellence Research Centre (BERC) program of the Basque Government. | es_ES |
dc.format | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier BV | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099857-B-I00/ES/INTERACCIONES MOLECULARES EN MEMBRANAS Y PAPEL DE LOS ESFINGOLIPIDOS EN PROCESOS DE DEGRADACION CELULAR: UN ENFOQUE BIOFISICO/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114755GB-C33/ES/SIMULACION COMPUTACIONAL Y CARACTERIZACION FOTOFISICA AVANZADA DE BIOSONDAS MOLECULARES INTELIGENTES/ | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Lipid bilayer phases | es_ES |
dc.subject | Nile red | es_ES |
dc.subject | Phospholipid rippled phases | es_ES |
dc.subject | FLIM | es_ES |
dc.subject | Fluorescence | es_ES |
dc.title | Liquid-crystalline, liquid-ordered, rippled and gel lipid bilayer phases as observed with nile red fluorescence | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.molliq.2022.119874 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.molliq.2022.119874 | es_ES |
dc.identifier.e-issn | 1873-3166 | - |
dc.rights.license | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011033 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Aparece en las colecciones: | (IBF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Liquid_crystalline_Sot.pdf | 6,11 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
1
checked on 03-may-2024
WEB OF SCIENCETM
Citations
1
checked on 28-feb-2024
Page view(s)
23
checked on 11-may-2024
Download(s)
32
checked on 11-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons