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Título: | Anti-syntaxin antibodies inhibit calcium-dependent catecholamine secretion from permeabilized chromaffin cells |
Autor: | Gutiérrez, Luis M. CSIC ORCID; Quintanar, José Luis; Viniegra, Salvador CSIC ORCID; Salinas, Eva María; Moya, Fernando CSIC; Reig, Juan Antonio | Fecha de publicación: | 1995 | Editor: | Elsevier | Citación: | Biochemical and Biophysical Research Communications 206(1): 1-7 (1995) | Resumen: | Adrenomedullary chromaffin cells release catecholamines in response to the intracellular calcium rise upon stimulation by different secretagogues. The presence of syntaxin 1, a protein presumably involved in docking of synaptic vesicles to presynaptic membranes, has been investigated in chromaffin cells. The study using two different monoclonal antibodies shows that syntaxin 1 is present in the chromaffin cell membrane fraction. Functional experiments demonstrate that anti-syntaxin antibodies inhibit calcium-dependent secretion in permeabilized cells. These results suggest that syntaxin 1 is an important component of the secretory machinery in chromaffin cells. | Versión del editor: | https://doi.org/10.1006/bbrc.1995.1001 | URI: | http://hdl.handle.net/10261/288580 | DOI: | 10.1006/bbrc.1995.1001 | ISSN: | 0006-291X |
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