Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/286001
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2 |
Autor: | Alcolea, Pedro J. CSIC ORCID ; Larraga, Jaime CSIC ORCID CVN ; Rodríguez-Martín, Daniel; Alonso, Ana CSIC ORCID ; Loayza, Francisco CSIC; Rojas, José Manuel; Ruiz-García, Silvia CSIC ORCID; Louloudes-Lázaro, Andrés; Carlón, Ana B.; Sánchez-Cordón, P. J.; Nogales-Altozano, Pablo; Redondo, Natalia CSIC ORCID ; Manzano, Miguel; Lozano, Daniel; Palomero, Jesús; Montoya, María CSIC ORCID ; Vallet-Regí, María; Martín, Verónica; Sevilla, Noemí CSIC ORCID ; Larraga, Vicente CSIC ORCID | Fecha de publicación: | 9-nov-2022 | Editor: | Frontiers Media | Citación: | Frontiers in Immunology 13: 1023255 (2022) | Resumen: | SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we present a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the non-replicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19. | Descripción: | 17 p.-8 fig. | Versión del editor: | https://doi.org/10.3389/fimmu.2022.1023255 | URI: | http://hdl.handle.net/10261/286001 | DOI: | 10.3389/fimmu.2022.1023255 | E-ISSN: | 1664-3224 |
Aparece en las colecciones: | (CIB) Artículos (INIA) Artículos (PTI Salud Global) Colección Especial COVID-19 |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
fimmu_alcolea_2022.pdf | Artículo principal | 6 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
4
checked on 10-abr-2024
WEB OF SCIENCETM
Citations
4
checked on 27-feb-2024
Page view(s)
97
checked on 27-abr-2024
Download(s)
46
checked on 27-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons