Molecular cloning and characterisation of the griffon vulture (Gyps fulvus) toll-like receptor 1

Abstract

The toll-like receptor (TLR) family is an ancient pattern recognition receptor family, conserved from insects to mammals. Members of the TLR family are vital to immune function through the sensing of pathogenic agents and initiation of an appropriate immune response. In this study, we cloned a cDNA encoding for a griffon vulture (Gyps fulvus) orthologue of mammalian TLR1 (CD281). The predicted 650 amino acid sequence comprised an extracellular domain with five leucine-rich repeats (LRR) and an LRR-C-terminal (LRR-CT) motif, followed by a 23 amino acid transmembrane segment, and a 190 amino acid intracytoplasmic region containing the Toll/IL-1R (TIR) domain. Vulture TLR1 and TIR domain showed 64% and 86% amino acid sequence similarity with chicken sequences. The tissue and cell expression pattern of vulture TLR1 were analysed by real time-PCR (RT-PCR) and correlated with the ability to respond to various pathogenic challenges. Despite the similarities in the overall structure and expression pattern of vulture TLR1 with other vertebrate TLRs, the length of the vulture TLR ectodomain, number and position of LRRs and N-glycosylation sites suggest structural differences that may have functional implications.