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http://hdl.handle.net/10261/282297
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Price, Daniel R.G. | es_ES |
dc.contributor.author | Küster, Tatiana | es_ES |
dc.contributor.author | Øines, Øivind | es_ES |
dc.contributor.author | Oliver, E. Margaret | es_ES |
dc.contributor.author | Bartley, Kathryn | es_ES |
dc.contributor.author | Nunn, Francesca | es_ES |
dc.contributor.author | Lima-Barbero, José Francisco | es_ES |
dc.contributor.author | Pritchard, James | es_ES |
dc.contributor.author | Karp-Tatham, Eleanor | es_ES |
dc.contributor.author | Hauge, Helena | es_ES |
dc.contributor.author | Blake, Damer P. | es_ES |
dc.contributor.author | Tomley, Fiona M. | es_ES |
dc.contributor.author | Nisbet, Alasdair J. | es_ES |
dc.date.accessioned | 2022-11-04T14:45:21Z | - |
dc.date.available | 2022-11-04T14:45:21Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Avian Pathology 48(sup. 1): S60-S74 (2019) | es_ES |
dc.identifier.issn | 1465-3338 | - |
dc.identifier.uri | http://hdl.handle.net/10261/282297 | - |
dc.description.abstract | Dermanyssus gallinae, the poultry red mite, is a global threat to the commercial egg-laying industry. Control of D. gallinae is difficult, with only a limited number of effective pesticides and non-chemical treatments available. Here, we characterize the candidate vaccine antigen D. gallinae cathepsin D-1 (Dg-CatD-1) and demonstrate that purified refolded recombinant Dg-Cat-D1 (rDg-CatD-1) is an active aspartyl proteinase which digests haemoglobin with a pH optimum of pH 4. Soluble protein extracts from D. gallinae also have haemoglobinase activity, with a pH optimum comparable to the recombinant protein, and both proteinase activities were inhibited by the aspartyl proteinase inhibitor Pepstatin A. Enzyme activity and the ubiquitous localization of Dg-CatD-1 protein in sections of adult female mites is consistent with Dg-CatD-1 being a lysosomal proteinase. Using Dg-CatD-1 as a model vaccine antigen, we compared vaccine delivery methods in laying hens via vaccination with: (i) purified rDg-CatD-1 with Montanide™ ISA 71 VG adjuvant; (ii) recombinant DNA vaccines for expression of rDg-CatD-1 and (iii) transgenic coccidial parasite Eimeria tenella expressing rDg-CatD-1. In two independent trials, only birds vaccinated with rDg-CatD-1 with Montanide™ ISA 71 VG produced a strong and long-lasting serum anti-rDg-Cat-D1 IgY response, which was significantly higher than that in control birds vaccinated with adjuvant only. Furthermore, we showed that egg-laying rates of D. gallinae mites fed on birds vaccinated with rDg-CatD-1 in Montanide™ ISA 71 VG was reduced significantly compared with mites fed on unvaccinated birds. | es_ES |
dc.description.sponsorship | This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under Grant Agreement No 635408 (“PARAGONE: vaccines for animal parasites”).This article, which is included in the ‘Research update on Dermanyssus gallinae, the poultry red mite’ supplement, is sponsored and supported by Coventry University. | es_ES |
dc.format | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Taylor & Francis | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/635408 | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | Evaluation of vaccine delivery systems for inducing long-lived antibody responses to Dermanyssus gallinae antigen in laying hens | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1080/03079457.2019.1612514 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.contributor.funder | Coventry University | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001313 | es_ES |
dc.identifier.pmid | 31032631 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
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