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Título: | Pdx1 is transcriptionally regulated by EGR-1 during nitric oxide-induced endoderm differentiation of mouse embryonic stem cells |
Autor: | Salguero-Aranda, Carmen CSIC ORCID; Beltrán-Povea, Amparo CSIC; Postigo-Corrales, Fátima CSIC; Hitos, Ana Belén; Díaz, Irene CSIC; Caballano-Infantes, Estefanía CSIC ORCID; Fraga, Mario F. CSIC ORCID; Hmadcha, Abdelkrim CSIC ORCID; Martín, Franz CSIC ORCID; Soria Escoms, Bernat CSIC ORCID; Tapia-Limonchi, Rafael CSIC ORCID; Bedoya Bergua, Francisco Javier CSIC ORCID; Tejedo Huamán, Juan Rigoberto CSIC ORCID | Palabras clave: | EGR-1 Endoderm differentiation Pdx1 mESCs Nitric oxide |
Fecha de publicación: | 2022 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 23(7): 3920 (2022) | Resumen: | The transcription factor, early growth response-1 (EGR-1), is involved in the regulation of cell differentiation, proliferation, and apoptosis in response to different stimuli. EGR-1 is described to be involved in pancreatic endoderm differentiation, but the regulatory mechanisms controlling its action are not fully elucidated. Our previous investigation reported that exposure of mouse embryonic stem cells (mESCs) to the chemical nitric oxide (NO) donor diethylenetriamine nitric oxide adduct (DETA-NO) induces the expression of early differentiation genes such as pancreatic and duodenal homeobox 1 (Pdx1). We have also evidenced that Pdx1 expression is associated with the release of polycomb repressive complex 2 (PRC2) and P300 from the Pdx1 promoter; these events were accompanied by epigenetic changes to histones and site-specific changes in the DNA methylation. Here, we investigate the role of EGR-1 on Pdx1 regulation in mESCs. This study reveals that EGR-1 plays a negative role in Pdx1 expression and shows that the binding capacity of EGR-1 to the Pdx1 promoter depends on the methylation level of its DNA binding site and its acetylation state. These results suggest that targeting EGR-1 at early differentiation stages might be relevant for directing pluripotent cells into Pdx1-dependent cell lineages. | Versión del editor: | https://doi.org/10.3390/ijms23073920 | URI: | http://hdl.handle.net/10261/279617 | DOI: | 10.3390/ijms23073920 | E-ISSN: | 1422-0067 |
Aparece en las colecciones: | (CINN) Artículos (CABIMER) Artículos (IBIS) Artículos |
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