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Open Access item Genome-Wide Profiling of p63 DNA–Binding Sites Identifies an Element that Regulates Gene Expression during Limb Development in the 7q21 SHFM1 Locus

Authors:Kouwenhoven, Evelyn N.
Van Heeringen, Simon J.
Tena, Juan J.
Oti, Martin
Dutilh, Bas E.
Alonso, M. Eva
Calle-Mustienes, Elisa de la
Smeenk, Leonie
Rinne, Tuula
Parsaulian, Lilian
Bolat, Emine
Jurgelenaite, Rasa
Huynen, Martijn A.
Hoischen, Alexander
Veltman, Joris A.
Brunner, Han G.
Roscioli, Tony
Oates, Emily
Wilson, Meredith
Manzanares, Miguel
Gómez-Skarmeta, José Luis
Stunnenberg, Hendrik G.
Lohrum, Marion
Van Bokhoven, Hans
Zhou, Huiqing
Issue Date:Aug-2010
Publisher:Public Library of Science
Citation:PLoS Genetics 6(8): e1001065 (2010)
Abstract:Heterozygous mutations in p63 are associated with split hand/foot malformations (SHFM), orofacial clefting, and ectodermal abnormalities. Elucidation of the p63 gene network that includes target genes and regulatory elements may reveal new genes for other malformation disorders. We performed genome-wide DNA–binding profiling by chromatin immunoprecipitation (ChIP), followed by deep sequencing (ChIP–seq) in primary human keratinocytes, and identified potential target genes and regulatory elements controlled by p63. We show that p63 binds to an enhancer element in the SHFM1 locus on chromosome 7q and that this element controls expression of DLX6 and possibly DLX5, both of which are important for limb development. A unique micro-deletion including this enhancer element, but not the DLX5/DLX6 genes, was identified in a patient with SHFM. Our study strongly indicates disruption of a non-coding cis-regulatory element located more than 250 kb from the DLX5/DLX6 genes as a novel disease mechanism in SHFM1. These data provide a proof-of-concept that the catalogue of p63 binding sites identified in this study may be of relevance to the studies of SHFM and other congenital malformations that resemble the p63-associated phenotypes.
Description:15 páginas, 5 figuras, 3 tablas.-- Información de apoyo: 8 figuras, 8 tablas.
Publisher version (URL):http://dx.doi.org/10.1371/journal.pgen.1001065
Appears in Collections:(CABD) Artículos

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