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Título: | Co-transfer of plasmid-encoded bla carbapenemases genes and mercury resistance operon in high-risk clones of Klebsiella pneumoniae |
Autor: | Pérez-Palacios, Patricia CSIC ORCID; Delgado-Valverde, Mercedes CSIC ORCID; Gual-de-Torrella, Ana CSIC ORCID; Oteo-Iglesias, Jesús; Pascual, Álvaro CSIC ORCID; Fernández-Cuenca, Felipe CSIC ORCID CVN | Palabras clave: | Klebsiella pneumoniae Carbapenemase gene Metals Mercury Co-resistance |
Fecha de publicación: | 20-nov-2021 | Editor: | Springer Nature | Citación: | Applied Microbiology and Biotechnology 105: 9231-9242 (2021) | Resumen: | Carbapenemase-producing Klebsiella pneumoniae (CP-Kp) is a real global health threat. Environmental reservoirs of resistance gene determinats, such as effluents of hospital wastewaters, are acquiring increased relevance in the selection of plasmid-encoded carbapenemase genes. The presence of Hg in environmental reservoirs may exert a positive selective pressure on tolerant bacteria, favoring the co-transfer of carbapenemase genes and mer operons. In our study, 63 CP-Kp isolates were screened for mer operons by whole genome sequencing (MySeq). Conjugation assays were performed with 24 out of 63 CP-Kp isolates harboring the mer operon. Ten transconjugants (Tc-Kp) were selected with Hg. Plasmid DNA of Tc-Kp was extracted and sequenced using single-molecule real-time (SMRT) technology (PacBio, Sequel II system) with later annotation. Plasmid analysis revealed that Tc-Kp from blaIMP-like (n = 3) showed a single plasmid belonging to IncC group with two complete mer operon next to blaIMP-like. Tc-Kp from blaVIM-1 (n = 2) harbored two plasmids, one with blaVIM-1 in an IncL, and mer operon was in an IncFIB plasmid. Tc-Kp from blaOXA-48-like (n = 5) showed 2 plasmids. blaOXA-48-like was found in an IncL plasmid, whereas mer operon was (i) in an IncR plasmid associated with blaCTX-M-15 in 3 Tc-Kp-OXA-48-like, (ii) in an IncC plasmid associated with blaCMY-2 in 1 Tc-Kp-OXA-48-like, (iii) and in an IncFIB plasmid associated with blaCTX-M-15 in 1 Tc-Kp-OXA-48-like. This is, to our knowledge, the first study to describe in K. pneumoniae producing plasmid-encoded carbapenemase, the potential impact of Hg in the co-transfer of mer operons and carbapenemase genes located in the same or different plasmids. | Versión del editor: | http://dx.doi.org/10.1007/s00253-021-11684-2 | URI: | http://hdl.handle.net/10261/269417 | DOI: | 10.1007/s00253-021-11684-2 | Identificadores: | doi: 10.1007/s00253-021-11684-2 issn: 0175-7598 e-issn: 1432-0614 |
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