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Título: | Stimulation of phosphoinositide hydrolysis by γ- and δ- hexachlorocyclohexane in primary cultures of cerebellar granule cells: Interaction with glutamate and carbachol receptor-mediated phosphoinositide response and effects of specific pharmacological agents |
Autor: | Sanfeliu, Coral CSIC ORCID; Rosa, Roser; Suñol, Cristina CSIC ORCID ; Rodríguez-Farré, Eduard; Cristòfol, Rosa CSIC | Fecha de publicación: | may-1996 | Editor: | Elsevier | Citación: | Pesticide Biochemistry and Physiology 55(1): 64-76 (1996) | Resumen: | The accumulation of [3H]inositol phosphates derived from phosphoinositide hydrolysis stimulated by γ- and δ-hexachlorocyclohexane isomers was characterized in primary cultures of cerebellar granule cells. The EC50 for γ- and δ-hexachlorocyclohexane was 106 and 85 μM, respectively. Stimulatory effects of hexachlorocyclohexane isomers were highly dependent on extracellular Ca2+ but they were not inhibited by classical voltage sensitive Ca2+ and Na+ channel blockers. The Na+/Ca2+ exchanger blocker amiloride caused a significant inhibition of δ- hexachlorocyclohexane effects. A lack of additive effects on phosphoinositide hydrolysis stimulation between hexachlorocyclohexane isomers and depolarization by high K+ was observed. The effects of each hexachlorocyclohexane isomer on glutamate or carbachol-induced inositol phosphate stimulation were also not additive, whereas that of high K+ was less than additive or synergistic when combined with glutamate or carbachol, respectively. When neuronal cells were exposed to the combination of δ- hexachlorocyclohexane and glutamate or carbachol in the presence of the respective receptor antagonists only the δ-hexachlorocyclohexane stimulatory effect was observed. Thus, the inhibition of glutamate- and carbachol- stimulated phosphoinositide hydrolysis by δ-hexachlorocyclohexane seems to imply a receptor-independent mechanism. It is suggested that both γ- and δ- hexachlorocyclohexane activate phosphoinositide-specific phospholipase C partly through Ca2+-related mechanisms. | Versión del editor: | http://dx.doi.org/10.1006/pest.1996.0036 | URI: | http://hdl.handle.net/10261/268784 | DOI: | 10.1006/pest.1996.0036 | Identificadores: | doi: 10.1006/pest.1996.0036 issn: 0048-3575 |
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