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Título: | Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis |
Autor: | Paz-Cedeno, Fernando Roberto; Carceller, Jose Miguel; Iborra Chornet, Sara; Donato, Ricardo Keitel; Godoy, Anna Paula; Veloso de Paula, Ariela; Monti, Rubens; Corma, Avelino CSIC ORCID ; Masarin, Fernando | Fecha de publicación: | feb-2021 | Citación: | Renewable Energy 164: 491-501 (2021) | Resumen: | For producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g.h) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production. | Versión del editor: | http://dx.doi.org/10.1016/j.renene.2020.09.059 | URI: | http://hdl.handle.net/10261/268351 | DOI: | 10.1016/j.renene.2020.09.059 | Identificadores: | doi: 10.1016/j.renene.2020.09.059 issn: 1879-0682 |
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