Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/255621
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Antiamoeboid activity of squamins C–F, cyclooctapeptides from Annona globifora |
Autor: | Rodríguez-Expósito, Rubén L.; Sosa-Rueda, Javier; Reyes-Batlle, María; Sifaoui, Inés; Cen-Pacheco, Francisco; Hernández Daranas, Antonio CSIC ORCID ; Díaz-Marrero, Ana R. CSIC ORCID; Piñero, José E.; Fernández, José J.; Lorenzo-Morales, Jacob | Palabras clave: | Squamin Cyclopeptide Acanthamoeba Annona Antiamoeboid |
Fecha de publicación: | 9-dic-2021 | Editor: | Elsevier Australian Society for Parasitology |
Citación: | International Journal for Parasitology: Drugs and Drug Resistance 17: 67-79 (2021) | Resumen: | Free-living amoebae of Acanthamoeba spp. are causative agents of human infections such as granulomatous amoebic encephalitis (GAE) and Acanthamoeba keratitis (AK). The exploration of innovative chemical entities from natural sources that induce intrinsic apoptotic pathway or a Programmed Cell Death (PCD) in Acanthamoeba protozoa is essential to develop new therapeutic strategies. In this work, the antiamoeboid activity of squamins C–F (1–4), four cyclooctapeptides isolated from Annona globiflora was tested in vitro against Acanthamoeba castellanii Neff, A. polyphaga, A. quina, and A. griffini, and a structure–activity relationship was also established. The most sensitive strain against all tested cyclooctapeptides was A. castellanii Neff being the R conformers of the S-oxo-methionine residue, squamins D (2) and F (4), the most active against the trophozoite stage. It is remarkable that all four peptides showed no cytotoxic effects against murine macrophages cell line J774A.1. The analysis of the mode of action of squamins C–F against A. castellanii indicate that these cyclopeptides induced the mechanisms of programmed cell death (PCD). All peptides trigger mitochondrial damages, significant inhibition of ATP production compared to the negative control, chromatin condensation and slight damages in membrane that affects its permeability despite it conserves integrity at the IC90 for 24 h. An increase in reactive oxygen species (ROS) was observed in all cases. | Versión del editor: | https://doi.org/10.1016/j.ijpddr.2021.08.003 | URI: | http://hdl.handle.net/10261/255621 | DOI: | 10.1016/j.ijpddr.2021.08.003 | ISSN: | 2211-3207 |
Aparece en las colecciones: | (IPNA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Antiamoeboid-Daranas-2021-InternationalJournalforParasitology.pdf | Artículo principal | 9,33 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
6
checked on 28-abr-2024
WEB OF SCIENCETM
Citations
4
checked on 22-feb-2024
Page view(s)
72
checked on 01-may-2024
Download(s)
86
checked on 01-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons