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Título: | Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast |
Autor: | Scheler, Ulschan; Brandt, Wolfgang; Porzel, Andrea; Rothe, Kathleen; Manzano, David CSIC ORCID; Božić, Dragana; Papaefthimiou, Dimitra; Ulrich Balcke, Gerd; Henning, Anja; Lohse, Swanhild; Marillonnet, Sylvestre; Kanellis, Angelos K.; Ferrer, Albert; Tissier, Alain | Fecha de publicación: | 2016 | Editor: | Springer Nature | Citación: | Nature Communications 7: 12942 (2016) | Resumen: | Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities. | Versión del editor: | https://doi.org/10.1038/ncomms12942 | URI: | http://hdl.handle.net/10261/248574 | DOI: | 10.1038/ncomms12942 | E-ISSN: | 2041-1723 |
Aparece en las colecciones: | (CRAG) Artículos |
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