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Título: | Chronic exposure of cultured transformed mouse epidermal cells to transforming growth factor-beta 1 induces an epithelial-mesenchymal transdifferentiation and a spindle tumoral phenotype |
Autor: | Caulín, Carlos CSIC; Scholl, Francisco G.; Frontelo, Pilar; Gamallo, Carlos; Quintanilla, Miguel CSIC ORCID | Fecha de publicación: | ago-1995 | Editor: | American Association for Cancer Research | Citación: | Cell Growth and Differentiation 6(8): 1027-1035 (1995) | Resumen: | Transformed mouse epidermal keratinocytes of the cell line PDV, when cultured under the presence of transforming growth factor-beta 1 (TGF-beta 1), escaped the block of growth exerted by this factor in normal keratinocytes and underwent marked changes in cell differentiation. TGF-beta 1 induced disruption of epithelial interactions, dispersion of cells, increased local movement, and conversion to a fibroblast-like morphology. These changes were reversible and correlated with down-regulation of epithelial protein markers such as E-cadherin and cytokeratins and upregulation of vimentin. TGF-beta 1-treated cells with a fibroblast-like phenotype induced spindle cell carcinomas upon transplantation in athymic nude mice, whereas untreated PDV cells or fusiform cells reverted to the epithelial phenotype and produced well-differentiated squamous cell carcinomas. Nontumorigenic immortalized epidermal keratinocytes, when grown under the presence of TGF-beta 1, did not transdifferentiate to a mesenchymal phenotype, their proliferation was blocked, and cells finally died. These results suggest a role of TGF-beta 1 in the progression of squamous carcinoma cells to spindle carcinomas in mouse skin carcinogenesis. | Descripción: | 9 pages, 7 figures, 1 table. | Versión del editor: | http://cgd.aacrjournals.org/cgi/content/abstract/6/8/1027 | URI: | http://hdl.handle.net/10261/24121 | ISSN: | 1044-9523 |
Aparece en las colecciones: | (IIBM) Artículos |
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