Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/241035
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Asbach, Benedikt | - |
dc.contributor.author | Kibler, K. | - |
dc.contributor.author | Köstler, Josef | - |
dc.contributor.author | Perdiguero, Beatriz | - |
dc.contributor.author | Yates, Nicole L. | - |
dc.contributor.author | Stanfield-Oakley, Sherry | - |
dc.contributor.author | Tomaras, Georgia D. | - |
dc.contributor.author | Kao, Shing-Fen | - |
dc.contributor.author | Foulds, Kathryn E. | - |
dc.contributor.author | Roederer, Mario | - |
dc.contributor.author | Seaman, Michael S. | - |
dc.contributor.author | Montefiori, David C. | - |
dc.contributor.author | Parks, Robert | - |
dc.contributor.author | Ferrari, Guido | - |
dc.contributor.author | Forthal, Donald N. | - |
dc.contributor.author | Phogat, Sanjay | - |
dc.contributor.author | Tartaglia, James | - |
dc.contributor.author | Barnett, Susan W. | - |
dc.contributor.author | Self, Steven G. | - |
dc.contributor.author | Gottardo, Raphael | - |
dc.contributor.author | Cristillo, Anthony D. | - |
dc.contributor.author | Weiss, Deborah E. | - |
dc.contributor.author | Galmin, Lindsey | - |
dc.contributor.author | Ding, Song | - |
dc.contributor.author | Heeney, Jonathan L. | - |
dc.contributor.author | Esteban, Mariano | - |
dc.contributor.author | Jacobs, Bertram L. | - |
dc.contributor.author | Pantaleo, Giuseppe | - |
dc.contributor.author | Wagner, Ralf | - |
dc.date.accessioned | 2021-05-18T09:00:25Z | - |
dc.date.available | 2021-05-18T09:00:25Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier | doi: 10.1128/JVI.01529-18 | - |
dc.identifier | issn: 0022-538X | - |
dc.identifier | e-issn: 1098-5514 | - |
dc.identifier.citation | Journal of Virology 93(3): e01529-18 (2019) | - |
dc.identifier.uri | http://hdl.handle.net/10261/241035 | - |
dc.description.abstract | The use of heterologous immunization regimens and improved vector systems has led to increases in immunogenicity of HIV-1 vaccine candidates in nonhuman primates. In order to resolve interrelations between different delivery modalities, three different poxvirus boost regimens were compared. Three groups of rhesus macaques were each primed with the same DNA vaccine encoding Gag, Pol, Nef, and gp140. The groups were then boosted with either the vaccinia virus strain NYVAC or a variant with improved replication competence in human cells, termed NYVAC-KC. The latter was administered either by scarification or intramuscularly. Finally, macaques were boosted with adjuvanted gp120 protein to enhance humoral responses. The regimen elicited very potent CD4+ and CD8+ T cell responses in a well-balanced manner, peaking 2 weeks after the boost. T cells were broadly reactive and polyfunctional. All animals exhibited antigen-specific humoral responses already after the poxvirus boost, which further increased following protein administration. Polyclonal reactivity of IgG antibodies was highest against HIV-1 clade C Env proteins, with considerable cross-reactivity to other clades. Substantial effector functional activities (antibody-dependent cell-mediated cytotoxicity and antibody-dependent cell-mediated virus inhibition) were observed in serum obtained after the last protein boost. Notably, major differences between the groups were absent, indicating that the potent priming induced by the DNA vaccine initially framed the immune responses in such a way that the subsequent boosts with NYVAC and protein led only to an increase in the response magnitudes without skewing the quality. This study highlights the importance of selecting the best combination of vector systems in heterologous prime-boost vaccination regimens. | - |
dc.description.sponsorship | This investigation was funded by the Bill & Melinda Gates Foundation Poxvirus T-Cell Vaccine Discovery Consortium (PTVDC) (38599). The Vaccine Immune Monitoring Centers (OPP1032144 and OPP1032325) and the Vaccine Immunology Statistical Center (OPP1032317), as part of the Collaboration for AIDS Vaccine Discovery (CAVD), were funded by the Bill & Melinda Gates Foundation. Novartis Vaccines received support for this work under contract number HHSN266200500007C from DAIDS-NIAID-NIH. | - |
dc.language | eng | - |
dc.publisher | American Society for Microbiology | - |
dc.rights | closedAccess | - |
dc.subject | DNA vaccine | - |
dc.subject | Gag-Pol-Nef | - |
dc.subject | NYVAC | - |
dc.subject | NYVAC-KC | - |
dc.subject | T cell responses | - |
dc.subject | Antibody responses | - |
dc.subject | gp140 | - |
dc.subject | Human immunodeficiency virus | - |
dc.subject | Nonhuman primates | - |
dc.subject | Vaccine | - |
dc.title | Priming with a Potent HIV-1 DNA Vaccine Frames the Quality of Immune Responses prior to a Poxvirus and Protein Boost | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1128/JVI.01529-18 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JVI.01529-18 | - |
dc.date.updated | 2021-05-18T09:00:26Z | - |
dc.contributor.funder | Bill & Melinda Gates Foundation | - |
dc.contributor.funder | National Institute of Allergy and Infectious Diseases (US) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100000060 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100000865 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100006492 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
Aparece en las colecciones: | (CNB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
67
checked on 13-may-2024
Download(s)
14
checked on 13-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.