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Título: | Nanomechanical detection of Escherichia coli infection by bacteriophage T7 using cantilever sensors |
Autor: | Mertens, Johann CSIC ORCID; Cuervo, Ana; Carrascosa, José L. CSIC ORCID | Fecha de publicación: | 2019 | Editor: | Royal Society of Chemistry (UK) | Citación: | Nanoscale 11: 17689-17698 (2019) | Resumen: | Viruses that infect bacteria (bacteriophages) are a promising alternative treatment for bacterial diseases, especially in the case of antibiotic resistance. Due to a renewed interest in phage therapies, development of rapid and specific detection methods for bacteria/bacteriophage interaction are gaining attention for proper diagnosis and treatment. This paper describes a new method to detect the interaction between Escherichia coli and bacteriophage T7 in a sensitive and quantitative way, using the nanomechanical motion of bacteria adhered to a cantilever surface. Our approach combines both deflection and dynamic frequency-domain characterization. The device was able to determine the viability of a low amount of living bacteria attached to the cantilever, and was used to monitor T7 interaction with E. coli over a wide range of virus concentrations up to 109 PFU ml−1. The nanomechanical assay described here requires no protein labeling and can be performed in a single reaction without additional reagents. The system was able to detect the interaction between a few thousand particles through the fluctuation of mechanical energy over a broad range of frequencies. The presented data provides the basis for more detailed studies of the sequence of molecular events that contribute to the motion of the device. | Versión del editor: | http://dx.doi.org/10.1039/c9nr05240b | URI: | http://hdl.handle.net/10261/240899 | DOI: | 10.1039/c9nr05240b | Identificadores: | doi: 10.1039/c9nr05240b issn: 2040-3364 e-issn: 2040-3372 |
Aparece en las colecciones: | (CNB) Artículos |
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