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Título: | Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page |
Autor: | Cogliati, Sara; Herranz, Fernando CSIC ORCID CVN; Ruiz-Cabello, Jesús; Enríquez, José Antonio | Palabras clave: | BlueNative page Mitochondria Supercomplexes Digitonin Detergent Liquid dispersions |
Fecha de publicación: | 2021 | Editor: | Elsevier | Citación: | Biochimica et Biophysica Acta - Bioenergetics 1862 (2021) | Resumen: | The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle. | Versión del editor: | http://dx.doi.org/10.1016/j.bbabio.2020.148332 | URI: | http://hdl.handle.net/10261/239118 | DOI: | 10.1016/j.bbabio.2020.148332 | Identificadores: | doi: 10.1016/j.bbabio.2020.148332 issn: 0005-2728 |
Aparece en las colecciones: | (CBM) Artículos (IQM) Artículos |
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