Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/236417
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Synthesis of β(1 → 3) and β(1 → 6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophila |
Autor: | Yañez-Ñeco, Claudia V.; Cervantes, Fadia V.; Amaya-Delgado, Lorena; Ballesteros Olmo, Antonio CSIC ORCID; Plou Gasca, Francisco José CSIC ORCID ; Arrizón, Javier | Palabras clave: | Dairy industry Galactooligosaccharids Lactose Pantoea anthophila Prebiotics Transgalactosylation Recombinantb-galactosidase |
Fecha de publicación: | 5-nov-2020 | Editor: | Elsevier | Citación: | Electronic Journal of Biotechnology 49: 14- 21 (2021) | Resumen: | Background: Milk whey, a byproduct of the dairy industry has a negative environmental impact, can be used as a raw material for added-value compounds such as galactooligosaccharides (GOS) synthesis by β-galactosidases. Results: B-gal42 from Pantoea anthophila strain isolated from tejuino belonging to the glycosyl hydrolase family GH42, was overexpressed in Escherichia coli and used for GOS synthesis from lactose or milk whey. Crude cell-free enzyme extracts exhibited high stability; they were employed for GOS synthesis reactions. In reactions with 400 g/L lactose, the maximum GOS yield was 40% (w/w) measured by HPAEC-PAD, corresponding to 86% of conversion. This enzyme had a strong predilection to form GOS with β(1 → 6) and β(1 → 3) galactosyl linkages. Comparing GOS synthesis between milk whey and pure lactose, both of them at 300 g/L, these two substrates gave rise to a yield of 38% (60% of lactose conversion) with the same product profile determined by HPAEC-PAD. Conclusions: B-gal42 can be used on whey (a cheap lactose source) to produce added value products such as galactooligosaccharides. How to cite: Yañez-Ñeco CV, Cervantes FV, Amaya-Delgado L, et al. Synthesis of β(1→3) and β(1→6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophila. Electron J Biotechnol 2020;49. https://dx.doi.org/10.1016/j.ejbt.2020.10.004 | Versión del editor: | http://dx.doi.org/10.1016/j.ejbt.2020.10.004 | URI: | http://hdl.handle.net/10261/236417 | DOI: | 10.1016/j.ejbt.2020.10.004 | ISSN: | 0717-3458 |
Aparece en las colecciones: | (ICP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Yanez_Synthesis_ofb_Electronic_Jr_Bio_2020.pdf | 691,73 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
19
checked on 29-abr-2024
WEB OF SCIENCETM
Citations
10
checked on 21-feb-2024
Page view(s)
89
checked on 30-abr-2024
Download(s)
83
checked on 30-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons