Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/219707
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples |
Autor: | Velden, Vincent H. J. van der; Flores-Montero, Juan; Pérez-Andrés, Martin CSIC ORCID CVN; Martín-Ayuso, Marta; Crespo, Oliver; Blanco, Elena; Kalina, Tomas; Philippé, J.; Bonroy, C.; Bie, Maaike de; Marvelde, Jeroen G. te; Teodosio, Cristina; Corral-Mateos, A.; Kanderová, Veronika; Burg, Mirjam van der; Van Hoof, Dennis; Dongen, J. J. M. van | Palabras clave: | Dried antibody tubes Flow cytometry Hematological malignancies Immune deficiencies |
Fecha de publicación: | 2019 | Editor: | Elsevier | Citación: | Journal of Immunological Methods 475: 112287 (2019) | Resumen: | Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the primary immune deficiency orientation tube (PIDOT). Both tubes were evaluated on normal and/or on patient samples, comparing the mean fluorescence intensity of specific lymphocyte populations. Our data show that the dried tubes and liquid counterparts give highly comparable staining results, particularly when analyzed in multidimensional plots. In addition, the use of dried tubes may result in a reduced staining variability between different samples and thereby contributes to the generation of more robust data. Therefore, by using ready-to-use reagents in a dried single test tube format, the laboratory efficiency and quality will be improved. | Versión del editor: | https://doi.org/10.1016/j.jim.2017.03.011 | URI: | http://hdl.handle.net/10261/219707 | DOI: | 10.1016/j.jim.2017.03.011 | ISSN: | 0022-1759 |
Aparece en las colecciones: | (IBMCC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
optimiPIDOT.pdf | 613,26 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
30
checked on 07-may-2024
WEB OF SCIENCETM
Citations
30
checked on 28-feb-2024
Page view(s)
104
checked on 15-may-2024
Download(s)
365
checked on 15-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons