Identification of Hexaploid wheat cultivars based on isozyme patterns

Abstract

The classification and identification of 38 wheat cultivars of Triticum aestivum L. and one cultivar of Triticum spelta L. have been carried out using polyacrylamide and starch gel electrophoresis. The analyses were carried out using different parts of dry kernels, specifically embryo plus scutellum, endosperm and 15-day-old seedling leaves. The isozymic systems studied were : peroxidases, malic dehydrogenase, alcohol dehydrogenase, acid and alkaline phosphatase in dry kernels ; cc-amylases in germinating kernel endosperms and phospho-glucose mutase and isomerase, esterases, leucin-aminopeptidase, glutamic oxaloacetic transaminase, malic dehydrogenase and peroxidases in 15-day-old seedling leaves. Not all the cultivars could be identified. One group had three cultivars, five groups had two cultivars, and 26 groups had only one cultivar. The classification obtained using the isozymic patterns and the simi- larity indexes did not show a clear relation to the ancestor-descendant relationships among related cultivars