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Título: | A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing |
Autor: | Kellner, Max J.; Ross, James J.; Schnabl, Jakob; Dekens, Marcus P. S.; Heinen, Robert; Grishkovskaya, Irina; Bauer, Benedikt; Stadlmann, Johannes; Menéndez-Arias, Luis CSIC ORCID ; Fritsche-Polanz, Robert; Traugott, Marianna; Seitz, Tamara; Zoufaly, Alexander; Födinger, Manuela; Wenisch, Christoph; Zuber, Johannes; Vienna Covid-19 Diagnostics Initiative (VCDI); Pauli, Andrea; Brennecke, Julius | Fecha de publicación: | 23-jul-2020 | Editor: | BioRxiv | Citación: | BioRxiv: 10.1101/2020.06.23.166397 (2020) | Resumen: | Global efforts to combat the Covid-19 pandemic caused by the beta coronavirus SARS-CoV-2 are currently based on RT-qPCR-based diagnostic tests. However, their high cost, moderate throughput and reliance on sophisticated equipment limit widespread implementation. Loop-mediated isothermal amplification after reverse transcription (RT-LAMP) is an alternative detection method that has the potential to overcome these limitations. Here we present a rapid, robust, highly sensitive and versatile RT-LAMP based SARS-CoV-2 detection assay. Our forty-minute procedure bypasses a dedicated RNA isolation step, is insensitive to carry-over contamination, and uses a hydroxynaphthol blue (HNB)-based colorimetric readout, which allows robust SARS-CoV-2 detection from various sample types. Based on this assay, we have substantially increased sensitivity and scalability by a simple nucleic acid enrichment step (bead-LAMP), established a pipette-free version for home testing (HomeDip-LAMP), and developed a version with open source enzymes that can be produced in any molecular biology setting. Our advanced, universally applicable RT-LAMP assay is a major step towards population-scale SARS-CoV-2 testing. | Versión del editor: | https://doi.org/10.1101/2020.06.23.166397 | URI: | http://hdl.handle.net/10261/216969 | ISSN: | 10.1101/2020.06.23.166397 |
Aparece en las colecciones: | (PTI Salud Global) Colección Especial COVID-19 (CBM) Artículos |
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A rapid, highly sensitive_Kellner.pdf | 10,03 MB | Adobe PDF | Visualizar/Abrir |
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