New Lanostanoids from the Fungus Ganoderma concinna

Abstract

Three new compounds, 5α-lanosta-7,9(11),24-triene-3β-hydroxy-26-al (1), 5α-lanosta-7,9(11),24-triene-15α-26-dihydroxy-3-one (2), and 8α,9α-epoxy-4,4,14α-trimethyl-3,7,11,15,20-pentaoxo-5α-pregnane (3), were isolated from Ganoderma concinna along with 12 known compounds. The structures of compounds 1 and 2 were determined on the basis of MS and NMR studies. The structure of 3 was determined by MS, NMR, and single-crystal X-ray diffraction. Compounds 1, 2, and 3 induce apoptosis in human promyelocytic leukemia HL-60 cells, as indicated by examining the morphological features of cells and detection of DNA fragmentation by gel electrophoresis.