Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/203745
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
Autor: | Lama, Raquel CSIC ORCID CVN; Pereiro, Patricia CSIC ORCID; Costa, M. M. CSIC ORCID; Encinar, José Antonio; Medina-Gali, Regla María; Lamas, J.; Leiro, J.; Figueras Huerta, Antonio CSIC ORCID ; Novoa, Beatriz CSIC ORCID | Palabras clave: | Philasterides dicentrarchi Turbot Nk-lysin Antimicrobial peptides Antiparasitic |
Fecha de publicación: | 2019 | Citación: | 3rd International Symposium on Fish & Shellfish Immunology (2019) | Resumen: | P. dicentrarchi is one of the most threatening pathogens for turbot aquaculture. This protozoan ciliate is a causative agent of scuticociliatosis, which is a disease with important economic consequences for the sector. Neither vaccines nor therapeutic treatments are commercially available to combat this infection. Numerous antimicrobial peptides (AMPs) have demonstrated broad-spectrum activity against bacteria, viruses, fungi, parasites and even tumor cells; an example is Nk-lysin (Nkl), which is an AMP belonging to the saposin-like protein (SAPLIP) family with an ability to interact with biological membranes. Following the recent characterization of turbot Nkl, an expression plasmid encoding Nkl was constructed and an anti-Nkl polyclonal antibody was successfully tested. Using these tools, we demonstrated that although infection did not clearly affect nkl mRNA expression, it induced changes at the protein level. Turbot Nkl had the ability to inhibit proliferation of the P. dicentrarchi parasite both in vivo and in vitro. Moreover, a shortened peptide containing the active core of turbot Nkl (Nkl71-100) was synthesized and showed high antiparasitic activity with a direct effect on parasite viability that probably occurred via membrane disruption. Therefore, the nkl gene may be a good candidate for genetic breeding selection of fish, and either the encoded peptide or its shortened analog is a promising antiparasitic treatment in aquaculture | Descripción: | 3rd International Symposium on Fish & Shellfish Immunology, June 16th-20th 2019, Las Palmas de Gran Canaria (Spain) | URI: | http://hdl.handle.net/10261/203745 |
Aparece en las colecciones: | (IIM) Comunicaciones congresos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
162
checked on 15-may-2024
Download(s)
23
checked on 15-may-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.