Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/198545
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Conjugal transfer of the virulence plasmid of Salmonella enterica is regulated by the leucine‐responsive regulatory protein and DNA adenine methylation |
Autor: | Camacho, Eva María CSIC ORCID; Casadesús, Josep CSIC ORCID | Fecha de publicación: | 2002 | Editor: | Wiley-Blackwell | Citación: | Molecular Microbiology 44(6): 1589-1598 (2002) | Resumen: | Host-encoded functions that regulate the transfer operon (tra) in the virulence plasmid of Salmonella enterica (pSLT) were identified with a genetic screen. Mutations that decreased tra operon expression mapped in the lrp gene, which encodes the leucine-responsive regulatory protein (Lrp). Reduced tra operon expression in an Lrp- background is caused by lowered transcription of the traJ gene, which encodes a transcriptional activator of the tra operon. Gel retardation assays indicated that Lrp binds a DNA region upstream of the traJ promoter. Deletion of the Lrp binding site resulted in lowered and Lrp-independent traJ transcription. Conjugal transfer of pSLT decreased 50-fold in a Lrp- background. When a FinO- derivative of pSLT was used, conjugal transfer from an Lrp- donor decreased 1000-fold. Mutations that derepressed tra operon expression mapped in dam, the gene encoding Dam methyltransferase. Expression of the tra operon and conjugal transfer remain repressed in an Lrp- Dam- background. These observations support the model that Lrp acts as a conjugation activator by promoting traJ transcription, whereas Dam methylation acts as a conjugation repressor by activating FinP RNA synthesis. This dual control of conjugal transfer may also operate in other F-like plasmids such as F and R100. | Versión del editor: | https://doi.org/10.1046/j.1365-2958.2002.02981.x | URI: | http://hdl.handle.net/10261/198545 | DOI: | 10.1046/j.1365-2958.2002.02981.x | ISSN: | 0950-382X 1365-2958 |
Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
36
checked on 20-abr-2024
SCOPUSTM
Citations
69
checked on 25-abr-2024
WEB OF SCIENCETM
Citations
69
checked on 26-feb-2024
Page view(s)
135
checked on 23-abr-2024
Download(s)
19
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.