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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Gavira Gallardo, J. A. | - |
dc.contributor.author | Jesús, W. de | - |
dc.contributor.author | Cámara-Artigas, Ana | - |
dc.contributor.author | López-Garriga, J. | - |
dc.contributor.author | García Ruiz, Juan Manuel | - |
dc.date.accessioned | 2009-11-17T09:08:03Z | - |
dc.date.available | 2009-11-17T09:08:03Z | - |
dc.date.issued | 2006-03 | - |
dc.identifier.citation | Acta Crystallographica Section F 62(3): 196-199 (2006) | en_US |
dc.identifier.issn | 1067-0696 | - |
dc.identifier.uri | http://hdl.handle.net/10261/18706 | - |
dc.description | 4 pages, 3 figures, 1 table. | en_US |
dc.description.abstract | Haemoglobin II is one of three haemoglobins present in the cytoplasm of the Lucina pectinata mollusc that inhabits the Caribbean coast. Using HBII purified from its natural source, crystallization screening was performed using the counter-diffusion method with capillaries of 0.2 mm inner diameter. Crystals of HbII suitable for data collection and structure determination were grown in the presence of agarose at 0.1%(w/v) in order to improve their quality. The crystals belong to the tetragonal space group P42212, with unit-cell parameters a = b = 73.92, c = 152.35 Å, and diffracted X-rays to a resolution of better than 2.0 Å. The asymmetric unit is a homodimer with a corresponding Matthews coefficient (VM) of 3.15 Å3 Da-1 and a solvent content of 61% by volume. | en_US |
dc.description.sponsorship | This work is supported in part by NIH (COBREIP20RR016439 and SCORE506GM08103-27). JAG was supported by the AndalusianRegional Government, Spain. We thank Dr Cartwright for reviewing the manuscript and Hispanoagar for their kind supply of agarose. | en_US |
dc.format.extent | 13824 bytes | - |
dc.format.mimetype | application/vnd.ms-excel | - |
dc.language.iso | eng | en_US |
dc.publisher | International Union of Crystallography | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Haemoglobin II | en_US |
dc.subject | Lucina pectinata | en_US |
dc.title | Capillary Crystallization and molecular replacement solution of Haemoglobin II of the Clam Lucina pectinata | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1107/S1744309106002648 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1107/S1744309106002648 | en_US |
dc.identifier.pmid | 16511300 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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