Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/182934
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity |
Autor: | Santos-Moriano, Paloma CSIC ORCID; Kidibule, Peter E; Míguez, Noa; Fernández Arrojo, Lucía; Ballesteros Olmo, Antonio CSIC ORCID; Fernández Lobato, María CSIC ORCID; Plou Gasca, Francisco José CSIC ORCID | Palabras clave: | Biocatalysis Glycosidases Chitinases Chitosanases Chitosan oligosaccharides Deacetylation degree Anti-inflammatory |
Fecha de publicación: | 30-abr-2019 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Catalysts 9(5): 405 (2019) | Resumen: | By controlled hydrolysis of chitosan or chitin with different enzymes, three types of chitooligosaccharides (COS) with MW between 0.2 and 1.2 kDa were obtained: fully deacetylated (fdCOS), partially acetylated (paCOS), and fully acetylated (faCOS). The chemical composition of the samples was analyzed by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and MALDI-TOF mass spectrometry. The synthesized fdCOS was basically formed by GlcN, (GlcN)2, (GlcN)3, and (GlcN)4. On the contrary, faCOS contained mostly GlcNAc, (GlcNAc)2 and (GlcNAc)3, while paCOS corresponded to a mixture of at least 11 oligosaccharides with different proportions of GlcNAc and GlcN. The anti-inflammatory activity of the three COS mixtures was studied by measuring their ability to reduce the level of TNF-α (tumor necrosis factor) in murine macrophages (RAW 264.7) after stimulation with a mixture of lipopolysaccharides (LPS). Only fdCOS and faCOS were able to significantly reduce the production of tumor necrosis factor (TNF)-α at 6 h after stimulation with lipopolysaccharides. | Descripción: | © The Author(s). | Versión del editor: | https://doi.org/10.3390/catal9050405 | URI: | http://hdl.handle.net/10261/182934 | DOI: | 10.3390/catal9050405 | ISSN: | 2073-4344 | E-ISSN: | 2073-4344 |
Aparece en las colecciones: | (ICP) Artículos (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Tailored_Santos_Art2019.pdf | 1,11 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
30
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
22
checked on 26-feb-2024
Page view(s)
225
checked on 29-abr-2024
Download(s)
352
checked on 29-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons