Effects of light intensity and addition of carotene rich Dunaliella salina live cells on growth and antioxidant activity of Solea senegalensis Kaup (1858) larval and metamorphic stages

Abstract

Senegal sole Solea senegalensis larval and metamorphic stages were exposed to a range of light intensities (200, 1000 and 2000 lx) in cultures with or without supplementation of b-carotenerich live Dunaliella salina cells. Antioxidant biomarkers such as superoxide dismutase (SOD), catalase (KAT), total glutathione peroxidase (t-GPX) and malondialdehyde (MDA) were determined in larval and metamorphic stages. Growth was not affected (P > 0 05) either by light intensity or D. salina supplementation. Survival after metamorphosis was also unaffected by D. salina supplementation (mean S.E. 81 0 2 5% against 80 6 2 9% those fed the control algal diet) or light intensity (mean S.E. 74 3 4 9% for 200 lx, 85 1 2 7% for 1000 lx and 82 8 5 2% for 2000 lx, respectively). Light intensity affected (P < 0 05) KAT and t-GPX throughout development. SOD was only affected in metamorphosing larvae. The highest KAT and t-GPX activities were detected when the lowest light intensity (200 lx) was used. Light had no effect (P > 0 05) on MDA at any stage. Supplementing the diet with D. salina did not affect SOD, KAT or t-GPX and there was no interaction (P > 0 05) with light intensity. MDA was the only biomarker whose activity was significantly (P < 0 05) reduced when D. salina was supplemented to the larval rearing tanks. The effect of D. salina supplementation was only detected in metamorphosing larvae, whose MDA levels were noticeably higher than in earlier stages. These results are evidence of the antiperoxidative effect of b-carotene from live algae in the larval rearing process of marine fishes.