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http://hdl.handle.net/10261/170153
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Pérez-Martín, José | es_ES |
dc.contributor.author | Lorenzo, Víctor de | es_ES |
dc.date.accessioned | 2018-09-25T10:55:17Z | - |
dc.date.available | 2018-09-25T10:55:17Z | - |
dc.date.issued | 1996-04-05 | - |
dc.identifier.citation | The Journal of Biological Chemistry 271:7899-7902 (1996) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/170153 | - |
dc.description | 5 p.-3 fig. | es_ES |
dc.description.abstract | In the presence of m-xylene, the protein XylR encoded by the TOL plasmid of Pseudomonas putida, activates the GraphicGraphic-dependent promoter Pu. Early activation stages involve the release of the intramolecular repression caused by the signal reception N-terminal (A domain) of XylR on the central module of the protein. A genetic approach has been followed to locate the specific segment within A domain of XylR that is directly responsible for its down-regulation in the absence of inducer, as compared to that involved in effector (m-xylene) binding. For this, a reporter Escherichia coli strain carrying a monocopy transcriptional fusion of Pu to lacZ was transformed with a collection of plasmids encoding equivalent truncated varieties of XylR, consisting of nested and internal deletions throughout the entire A domain. Examination of the resulting phenotypes allowed the assignment of the A domain region near the central activation domain, as the portion of the protein responsible for the specific repression of XylR activity in the absence of m-xylene. | es_ES |
dc.description.sponsorship | This work was supported by Grant BIO95-0788 of the Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT) and Contract BIOTECH BIO2-CT92-0084 of the European Union. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Tol plasmid | es_ES |
dc.subject | Nucleotide-sequence | es_ES |
dc.subject | Promoters | es_ES |
dc.subject | Family | es_ES |
dc.title | Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1074/jbc.271.14.7899 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/ 10.1074/jbc.271.14.7899 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.contributor.orcid | Pérez-Martín, José [0000-0001-9849-7382] | es_ES |
dc.contributor.orcid | Lorenzo, Víctor de [0000-0002-6041-2731] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Fichero | Descripción | Tamaño | Formato | |
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J. Biol. Chem.-1996-Pérez-Martín-7899-902.pdf | Artículo principal | 71,79 kB | Adobe PDF | Visualizar/Abrir |
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