Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/168917
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Bioconjugation through mesitylene thiol alkylation |
Autor: | Ramos-Tomillero, Iván; Pérez-Chacón, Gema CSIC ORCID; Somovilla-Crespo, Beatriz; Sánchez-Madrid, Francisco; Domínguez, Juan Manuel; Cuevas, Carmen; Zapata, Juan M. CSIC ORCID ; Rodríguez, Hortensia; Albericio, Fernando CSIC ORCID | Fecha de publicación: | 2018 | Editor: | American Chemical Society | Citación: | Bioconjugate Chemistry 29(4): 1199-1208 (2018) | Resumen: | The design and generation of complex multifunctional macromolecular structures by bioconjugation is a hot topic due to increasing interest in conjugates with therapeutic applications. In this regard, the development of efficient, selective, and safe conjugation methods is a major objective. In this report, we describe the use of the bis(bromomethyl)benzene scaffold as a linker for bioconjugation with special emphasis on antibody conjugation. We first performed the monothioalkylation of 1,3,5-tris(bromomethyl)benzene, which rendered the reactive dibromotrimethylbenzyl derivatives to be used in thiol bis-alkylation. Next, we introduced into the linker either a bis(Cys)-containing peptide or anti-CD4 and -CD13 monoclonal antibodies, previously subjected to partial reduction of disulfide bonds. Mass spectrometry, UV–vis spectra, and SDS-PAGE experiments revealed that this bis-alkylating agent for bioconjugation preserved both antibody integrity and antibody–antigen binding affinity, as assessed by flow cytometry. Taken together, our results show that the mesitylene scaffold is a suitable linker for thiol-based bioconjugation reactions. This linker could be applicable in the near future for the preparation of antibody drug conjugates. | Versión del editor: | https://doi.org/10.1021/acs.bioconjchem.7b00828 | URI: | http://hdl.handle.net/10261/168917 | DOI: | 10.1021/acs.bioconjchem.7b00828 | Identificadores: | doi: 10.1021/acs.bioconjchem.7b00828 e-issn: 1520-4812 issn: 1043-1802 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
BioconManuscript.pdf | 578,63 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
2
checked on 10-may-2024
SCOPUSTM
Citations
5
checked on 09-may-2024
WEB OF SCIENCETM
Citations
5
checked on 28-feb-2024
Page view(s)
413
checked on 13-may-2024
Download(s)
636
checked on 13-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.