Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/166404
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Primer-Independent DNA Synthesis by a Family B DNA Polymerase from Self-Replicating Mobile Genetic Elements |
Autor: | Redrejo-Rodríguez, Modesto CSIC ORCID ; Ordóñez, Carlos D.; Berjón-Otero, Mónica; Aparicio-Maldonado, Cristian; Forterre, Patrick; Salas, Margarita CSIC ORCID ; Krupovic, Mart | Fecha de publicación: | 7-nov-2017 | Citación: | Cell Reports 21: 1574- 1587 (2017) | Resumen: | Family B DNA polymerases (PolBs) play a central role during replication of viral and cellular chromosomes. Here, we report the discovery of a third major group of PolBs, which we denote primer-independent PolB (piPolB), that might be a link between the previously known protein-primed and RNA/DNA-primed PolBs. PiPolBs are encoded by highly diverse mobile genetic elements, pipolins, integrated in the genomes of diverse bacteria and also present as circular plasmids in mitochondria. Biochemical characterization showed that piPolB displays efficient DNA polymerization activity that can use undamaged and damaged templates and is endowed with proofreading and strand displacement capacities. Remarkably, the protein is also capable of template-dependent de novo DNA synthesis, i.e., DNApriming activity, thereby breaking the long-standing dogma that replicative DNA polymerases require a pre-existing primer for DNA synthesis. We suggest that piPolBs are involved in self-replication of pipolins and may also contribute to bacterial DNA damage tolerance | URI: | http://hdl.handle.net/10261/166404 | DOI: | 10.1016/j.celrep.2017.10.039 | Identificadores: | doi: 10.1016/j.celrep.2017.10.039 issn: 2211-1247 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
SalasM_Primer-IndependentDNASynthesis.pdf | 4,56 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
15
checked on 27-abr-2024
SCOPUSTM
Citations
22
checked on 18-may-2024
WEB OF SCIENCETM
Citations
20
checked on 28-feb-2024
Page view(s)
253
checked on 21-may-2024
Download(s)
210
checked on 21-may-2024