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Closed Access item SADB phosphorylation of γ-tubulin regulates centrosome duplication

Authors:Alvarado-Kristensson, María
Rodríguez, María Josefa
Silió, Virginia
Valpuesta, José M.
Carrera, Ana C.
Issue Date:2-Aug-2009
Publisher:Nature Publishing Group
Citation:Nature Cell Biology 11(9): 1081-1092 (2009)
Abstract:Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose activity fluctuates during the cell cycle, localizes to centrosomes, and controls centrosome duplication. Reduction of endogenous SADB levels diminished centrosome numbers, whereas enhanced SADB expression induced centrosome amplification. SADB exerted this action through phosphorylation of γ-tubulin on Ser 131, as expression of a phosphomimetic Ser 131-to-Asp γ-tubulin mutant alone increased centrosome numbers, whereas non-phosphorylatable Ala 131-γ-tubulin impaired centrosome duplication. We propose that SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of γ-tubulin.
Description:17 pages, 11 figures.-- PMID: 19648910 [PubMed].-- Printed version published Sep 2009.
Supporting information available at: http://www.nature.com/ncb/journal/vaop/ncurrent/suppinfo/ncb1921_S1.html
Publisher version (URL):http://dx.doi.org/10.1038/ncb1921
URI:http://hdl.handle.net/10261/16397
ISSN:1465-7392 (Print)
1476-4679 (Online)
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Appears in Collections:(CNB) Artículos

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