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Título: | Sgs1’s roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6 |
Autor: | Bermúdez-López, Marcelino; Villoria, María Teresa CSIC; Esteras, Miguel; Jarmuz, Adam; Torres-Rosell, Jordi; Clemente-Blanco, Andrés CSIC ORCID CVN; Aragón, Luis | Palabras clave: | Sgs1–Top3 Smc5/6 Smc complexes Genome stability Homologous recombination |
Fecha de publicación: | 2016 | Editor: | Cold Spring Harbor Laboratory Press | Citación: | Genes and Development 30(11): 1339-1356 (2016) | Resumen: | The RecQ helicase Sgs1 plays critical roles during DNA repair by homologous recombination, fromend resection to Holliday junction (HJ) dissolution. Sgs1 has both pro- and anti-recombinogenic roles, and therefore its activity must be tightly regulated. However, the controls involved in recruitment and activation of Sgs1 at damaged sites are unknown. Here we show a two-step role for Smc5/6 in recruiting and activating Sgs1 through SUMOylation. First, auto-SUMOylation of Smc5/6 subunits leads to recruitment of Sgs1 as part of the STR (Sgs1–Top3–Rmi1) complex, mediated by two SUMO-interacting motifs (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6. Second, Smc5/6-dependent SUMOylation of Sgs1 and Top3 is required for the efficient function of STR. Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMΔ) or SUMO-dead alleles (sgs1-KR) exhibit unprocessed HJs at damaged replication forks, increased crossover frequencies during double-strand break repair, and severe impairment in DNA end resection. Smc5/6 is a key regulator of Sgs1’s recombination functions. | Versión del editor: | https://doi.org/10.1101/gad.278275.116 | URI: | http://hdl.handle.net/10261/157084 | DOI: | 10.1101/gad.278275.116 | Identificadores: | doi: 10.1101/gad.278275.116 e-issn: 1549-5477 issn: 0890-9369 |
Aparece en las colecciones: | (IBFG) Artículos |
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Sgsumo.pdf | 2,05 MB | Adobe PDF | Visualizar/Abrir |
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