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| Título: | Different Bla-g T cell antigens dominate responses in asthma versus rhinitis subjects |
Autor: | Dillon, M. B. C.; Bellés, Xavier CSIC ORCID ; Piulachs, Maria-Dolors CSIC ORCID ; Sette, Alessandro | Palabras clave: | Asthma CD4(+) T-cells Cockroach allergy Epitope |
Fecha de publicación: | dic-2015 | Editor: | Wiley-Blackwell | Citación: | Clinical and Experimental Allergy 45(12): 1856-1867 (2015) | Resumen: | [Background and objective] The allergenicity of several German cockroach (Bla-g) antigens at the level of IgE responses is well established. However, less is known about the specificity of CD4+ TH responses, and whether differences exist in associated magnitude or cytokine profiles as a function of disease severity. [Methods] Proteomic and transcriptomic techniques were used to identify novel antigens recognized by allergen-specific T cells. To characterize different TH functionalities of allergen-specific T cells, ELISPOT assays with sets of overlapping peptides covering the sequences of known allergens and novel antigens were employed to measure release of IL-5, IFNγ, IL-10, IL-17 and IL-21. [Results] Using these techniques, we characterized TH responses in a cohort of adult Bla-g-sensitized subjects, either with (n = 55) or without (n = 17) asthma, and nonsensitized controls (n = 20). T cell responses were detected for ten known Bla-g allergens and an additional ten novel Bla-g antigens, representing in total a 5-fold increase in the number of antigens demonstrated to be targeted by allergen-specific T cells. Responses of sensitized individuals regardless of asthma status were predominantly TH2, but higher in patients with diagnosed asthma. In asthmatic subjects, Bla-g 5, 9 and 11 were immunodominant, while, in contrast, nonasthmatic-sensitized subjects responded mostly to Bla-g 5 and 4 and the novel antigen NBGA5. [Conclusions] Asthmatic and nonasthmatic cockroach-sensitized individuals exhibit similar TH2-polarized responses. Compared with nonasthmatics, however, asthmatic individuals have responses of higher magnitude and different allergen specificity. |
Descripción: | Dillon, M. B. C. et al. | Versión del editor: | http://dx.doi.org/10.1111/cea.12643 | URI: | http://hdl.handle.net/10261/150609 | DOI: | 10.1111/cea.12643 | ISSN: | 0954-7894 | E-ISSN: | 1365-2222 |
| Aparece en las colecciones: | (IBE) Artículos |
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