Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/149845
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158 |
Autor: | Boer, Roeland CSIC ORCID; Ruiz-Masó, José A. CSIC ORCID ; Rueda, Manuel; Petoukhov, Maxim V.; Machón, Cristina CSIC ORCID ; Svergun, Dmitri I.; Orozco, Modesto; Solar, Gloria del CSIC ORCID ; Coll, Miquel CSIC ORCID | Fecha de publicación: | 15-feb-2016 | Editor: | Nature Publishing Group | Citación: | Scientific Reports 6:20915 (2016) | Resumen: | DNA replication initiation is a vital and tightly regulated step in all replicons and requires an initiator factor that specifically recognizes the DNA replication origin and starts replication. RepB from the promiscuous streptococcal plasmid pMV158 is a hexameric ring protein evolutionary related to viral initiators. Here we explore the conformational plasticity of the RepB hexamer by i) SAXS, ii)sedimentation experiments, iii) molecular simulations and iv) X-ray crystallography. Combining these techniques, we derive an estimate of the conformational ensemble in solution showing that the C-terminal oligomerisation domains of the protein form a rigid cylindrical scaffold to which the N-terminal DNA-binding/catalytic domains are attached as highly flexible appendages, featuring multiple orientations. In addition, we show that the hinge region connecting both domains plays a pivotal role in the observed plasticity. Sequence comparisons and a literature survey show that this hinge region could exists in other initiators, suggesting that it is a common, crucial structural element for DNA binding and manipulation. | Descripción: | 13 p.-7 fig.-1 tab. | Versión del editor: | http://dx.doi.org/10.1038/srep20915 | URI: | http://hdl.handle.net/10261/149845 | DOI: | 10.1038/srep20915 | ISSN: | 2045-2322 | E-ISSN: | 2045-2322 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
srep2016.pdf | Artículo principal | 1,97 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 20-abr-2024
SCOPUSTM
Citations
12
checked on 25-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 27-feb-2024
Page view(s)
405
checked on 23-abr-2024
Download(s)
223
checked on 23-abr-2024