Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/147139
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Proteomics characterization of tick-host-pathogen interactions |
Autor: | Popara, Marina CSIC; Villar, Margarita CSIC ORCID; Fuente, José de la CSIC ORCID | Palabras clave: | Protein extraction: Mass spectrometry Proteomics Electrophoresis LC-MS/MS Tick-host-pathogen interactions |
Fecha de publicación: | 2015 | Editor: | Springer Nature | Citación: | Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: 513-527 (2015) | Serie: | Methods in Molecular Biology 1247 | Resumen: | Ticks are blood-feeding arthropod ectoparasites of wild and domestic animals that transmit disease-causing pathogens to humans and animals worldwide and a good model for the characterization of tick-host-pathogen interactions. Tick-host-pathogen interactions consist of dynamic processes involving genetic traits of hosts, pathogens, and ticks that mediate their development and survival. Proteomics provides information on the protein content of cells and tissues that may differ from results at the transcriptomics level and may be relevant for basic biological studies and vaccine antigen discovery. In this chapter, we describe various methods for protein extraction and for proteomics analysis in ticks based on one-dimensional gel electrophoresis to characterize tick-host-pathogen interactions. Particularly relevant for this characterization is the use of blood-fed ticks. Therefore, we put special emphasis on working with replete ticks collected after feeding on vertebrate hosts. | URI: | http://hdl.handle.net/10261/147139 | DOI: | 10.1007/978-1-4939-2004-4_34 | Identificadores: | doi: 10.1007/978-1-4939-2004-4_34 isbn: 978-1-4939-2003-7 |
Aparece en las colecciones: | (IREC) Libros y partes de libros |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
229
checked on 23-abr-2024
Download(s)
100
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.