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Título:	Microbial encapsulation in monodisperse hydrogel microspheres enables fast and sensitive phenotypic analyses using flow cytometers
Autor:	Ramos, L. D.; Rodríguez-Aguilera, Juan Carlos CSIC ORCID; Cebolla, Ángel
Fecha de publicación:	2012
Citación:	22nd IUBMB and 37th FEBS (2012)
Resumen:	Detection and characterization of microorganisms by classical procedures usually involves culture during more than 20 generations in order to achieve the formation of macrocolonies on solid media. This can be impractical when fast analysis of growth is required or when the organisms are not cultivable. To overcome these problems, new technologies based on single-cell encapsulation have been developed. Thereby, microencapsulation allows the detection of microbial growth facilitating the isolation, the analysis and the classification of microscopic colonies by monitoring their development from encapsulated individual cells. Microbial proliferation inside the microcapsules can be detected using flow cytometry without requiring fluorescent labelling provided that the population of microparticles exhibits appropriate optical and mechanical properties and is monodisperse in size and shape. With this results we show the successful analysis of Saccharomyces Cerevisiae colonies using a Cellena Flow Focusing microencapsulator combining with flow cytometry. Moreover, we demonstrate the capacity of select a specific population of cells from a total one using the COPAS SELECT flow cytometer sorting system (Union Biometrica). In addition, these technologies let us to encapsule bacteria and human stem cells and can be used for a variety of applications: from characterizing secreted enzymes to detection of thermosensitive mutants. Alginate particle sizes were reproducibly selected from <100 lm to over 600 lm, by just replacing the disposable nozzle. Sterility was preserved during the microencapsulation procedure, preventing undesired contaminations.
Descripción:	Resumen del póster presentado al 22nd IUBMB & 37th FEBS Congress, celebrado en Sevilla (España) del 4 al 9 de septiembre de 2012.-- et al.
URI:	http://hdl.handle.net/10261/130145
Aparece en las colecciones:	(CABD) Comunicaciones congresos