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Título: | Preparation of N2, N2,7-trimethylguanosine affinity columns |
Autor: | Espuny, Ruth; Bahia, Diana; Barretto Cicarelli, Regina Maria; Codony, Carles; Khaouja, Amina; Aviñó, Anna CSIC ORCID; Eritja Casadellà, Ramón CSIC ORCID ; Bach-Elias, Montse CSIC ORCID | Fecha de publicación: | 1999 | Editor: | Taylor & Francis | Citación: | Nucleosides and Nucleotides 18(1): 125-136 (1999) | Resumen: | 2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG- binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs. | Versión del editor: | http://dx.doi.org/10.1080/07328319908045599 | URI: | http://hdl.handle.net/10261/109184 | DOI: | 10.1080/07328319908045599 | Identificadores: | doi: 10.1080/07328319908045599 issn: 0732-8311 |
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