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Título: | Nuclear bodies domain changes with microspore reprogramming to embryogenesis |
Autor: | Seguí-Simarro, José María CSIC ORCID; Bárány, Ivett CSIC; Suárez, R.; Fadón, Begoña; Testillano, Pilar S. CSIC ORCID ; Risueño, María Carmen CSIC | Palabras clave: | Microspore embryogenesis Nuclear architecture Nuclear bodies Cajal bodies Brassica napus Capsicum annuum Confocal microscopy Transmission electron microscopy |
Fecha de publicación: | ene-2006 | Editor: | Società Italiana di Istochimica | Citación: | European Journal of Histochemistry 50(1):35-44(2006) | Resumen: | We analysed the presence of nuclear bodies and particularly Cajal bodies during representative stages of gametophytic and haploid embryogenic development in isolated microspore and anther cultures of a model system (Brassica napus cv. Topas) and a recalcitrant species (Capsicum annuum L. var. Yolo Wonder B). The nuclear bodies domain is involved on several important roles on nuclear metabolism, and Cajal bodies are specifically involved on the storage and maturation of both snRNPs and snoRNPs, as well as other splicing factors, necessary for mRNA and pre-rRNA processing, but not directly on the transcription. In this study, immunofluorescence and immunogold labelling with anti-trimethylguanosine antibodies against the specific cap of snRNAs, ultrastructural and cytochemical analysis were performed on cryoprocessed samples at confocal and electron microscopy respectively. Results showed that Cajal bodies increase during the early stages of microspore embryogenic development (young pro-embryos), compared to microspore and pollen development. Our results suggest that Cajal bodies may have a role in the transcriptionally active, proliferative stages that characterise early microspore embryogenic development. | Descripción: | 10 pages, 5 figures.-- PMID: 16584983 [PubMed]. | Versión del editor: | http://www.ejh.it/Article.php?-recid=4023 | URI: | http://hdl.handle.net/10261/10612 | ISSN: | 1121-760X |
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