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Nuclear bodies domain changes with microspore reprogramming to embryogenesis

AuthorsSeguí-Simarro, José María ; Bárány, Ivett ; Suárez, R.; Fadón, Begoña; Testillano, P. S. ; Risueño, María Carmen
KeywordsMicrospore embryogenesis
Nuclear architecture
Nuclear bodies
Cajal bodies
Brassica napus
Capsicum annuum
Confocal microscopy
Transmission electron microscopy
Issue DateJan-2006
PublisherSocietà Italiana di Istochimica
CitationEuropean Journal of Histochemistry 50(1):35-44(2006)
AbstractWe analysed the presence of nuclear bodies and particularly Cajal bodies during representative stages of gametophytic and haploid embryogenic development in isolated microspore and anther cultures of a model system (Brassica napus cv. Topas) and a recalcitrant species (Capsicum annuum L. var. Yolo Wonder B). The nuclear bodies domain is involved on several important roles on nuclear metabolism, and Cajal bodies are specifically involved on the storage and maturation of both snRNPs and snoRNPs, as well as other splicing factors, necessary for mRNA and pre-rRNA processing, but not directly on the transcription. In this study, immunofluorescence and immunogold labelling with anti-trimethylguanosine antibodies against the specific cap of snRNAs, ultrastructural and cytochemical analysis were performed on cryoprocessed samples at confocal and electron microscopy respectively. Results showed that Cajal bodies increase during the early stages of microspore embryogenic development (young pro-embryos), compared to microspore and pollen development. Our results suggest that Cajal bodies may have a role in the transcriptionally active, proliferative stages that characterise early microspore embryogenic development.
Description10 pages, 5 figures.-- PMID: 16584983 [PubMed].
Publisher version (URL)http://www.ejh.it/Article.php?-recid=4023
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