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dc.contributor.authorMontilla, Antonia-
dc.contributor.authorRuiz-Matute, Ana I.-
dc.contributor.authorCorzo, Nieves-
dc.contributor.authorGiacomini, Cecilia-
dc.contributor.authorIrazoqui, Gabriela-
dc.date.accessioned2014-07-16T09:49:07Z-
dc.date.available2014-07-16T09:49:07Z-
dc.date.issued2013-
dc.identifierdoi: 10.1021/jf403321r-
dc.identifierissn: 0021-8561-
dc.identifiere-issn: 1520-5118-
dc.identifier.citationJournal of Agricultural and Food Chemistry 61(43): 10360-10367 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/99979-
dc.description.abstractChitooligosaccharides possessing remarkable biological properties can be obtained by enzymatic hydrolysis of chitin. In this work, the chitosanase activity of soluble and immobilized glycosyltransferase (Branchzyme) toward chitosan and biochemical characterization are described for the first time. This enzyme was found to be homotetrameric with a molecular weight of 256 kDa, an isoelectric point of 5.3, and an optimal temperature range of between 50 and 60 °C. It was covalently immobilized to glutaraldehyde–agarose with protein and activity immobilization yields of 67% and 17%, respectively. Immobilization improved enzyme stability, increasing its half-life 5-fold, and allowed enzyme reuse for at least 25 consecutive cycles. The chitosanase activity of Branchzyme on chitosan was similar for the soluble and immobilized forms. The reaction mixture was constituted by chitooligosaccharides with degrees of polymerization of between 2 and 20, with a higher concentration having degrees of polymerization of 3–8.-
dc.description.sponsorshipThis work has been financed by the R + D program of the Spanish Ministry of Innovation and Science (projects AGL2008/00941/ALI), Consolider Ingenio 2010 (FUNC-CFOOD CSD2007-00063), Cooperation Programme between C.S.I.C. (Spain) and Universidad de la República (Uruguay) 2009UY0013, and the “Programa de Desarrollo de las Ciencias Básicas” (PEDECIBA). A.I.R.-M. gratefully acknowledges a JAEDoc contract from C.S.I.C.-
dc.publisherAmerican Chemical Society-
dc.relation.isversionofPostprint-
dc.rightsopenAccess-
dc.subjectImmobilization-
dc.subjectBranchzyme-
dc.subjectCos-
dc.subjectChitooligosaccharides-
dc.subjectHPLC−SEC-
dc.subjectGC−FID-
dc.subjectMALDI-TOF-
dc.titleEnzymatic generation of chitooligosaccharides from chitosan using soluble and immobilized glycosyltransferase (Branchzyme)-
dc.typeartículo-
dc.identifier.doi10.1021/jf403321r-
dc.relation.publisherversionhttps://doi.org/10.1021/jf403321r-
dc.date.updated2014-07-16T09:49:07Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderUniversidad de la República (Uruguay)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderUniversidad de la República (Uruguay)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100006048es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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