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Título

Ultraviolet radiation dose calculation for algal suspensions using UVA and UVB extinction coefficients

Autor Navarro, Enrique ; Muñiz, Selene ; Korkaric, Muris; Wagner, Bettina; Cáceres, Miquel de; Behra, Renata
Palabras clave Chlamydomonas reinhardtii
UVR extinction
Synechocystis
Spectroradiometer
Radiometer
Algal cultures
Fecha de publicación 2014
EditorElsevier
Citación Journal of Photochemistry and Photobiology B: Biology 132(5): 94-101 (2014)
ResumenAlthough the biological importance of ultraviolet light (UVR) attenuation has been recognised in marine and freshwater environments, it is not generally considered in in vitro ecotoxicological studies using algal cell suspensions. In this study, UVA and UVB extinction were determined for cultures of algae with varying cell densities, and the data were used to calculate the corresponding extinction coefficients for both UVA and UVB wavelength ranges. Integrating the Beer-Lambert equation to account for changes in the radiation intensity reaching each depth, from the surface until the bottom of the experimental vessel, we obtained the average UVA and UVB intensity to which the cultured algal cells were exposed. We found that UVR intensity measured at the surface of Chlamydomonas reinhardtii cultures lead to a overestimation of the UVR dose received by the algae by 2-40 times. The approach used in this study allowed for a more accurate estimation of UVA and UVB doses. © 2014 Elsevier B.V. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.jphotobiol.2014.02.005
URI http://hdl.handle.net/10261/99947
DOI10.1016/j.jphotobiol.2014.02.005
Identificadoresdoi: 10.1016/j.jphotobiol.2014.02.005
issn: 1011-1344
e-issn: 1873-2682
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