English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/99902
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

In search of molecular markers for androgenesis

AuthorsPulido Bosch, Antonio; Castillo Alonso, Ana María ; Vallés Brau, María Pilar ; Olmedilla, Adela
KeywordsPectins
Callose
Barley
Arabinogalactan proteins
Anther culture
Androgenesis
Issue DateFeb-2002
PublisherSlovak Academic Press
CitationBiologia - Section Botany 57 (1): 29-36 (2002)
AbstractWe have used specific antibodies against callose, pectins and arabinogalactan-proteins, all components of the cell wall involved in embryo development, to study their distribution during androgenesis induced in barley anthers in the hope that they might serve as markers of this process. Callose was found in the outer region of the intine in induced embryos and in the numerous plasmodesmata within these embryos. Anti-callose antibodies proved useful for following the evolution of these structures throughout the formation of the pollen embryo. The monoclonal antibodies JIM5 and JIM7, which recognise unesterified and methylesterified pectins respectively, were used to follow the evolution of the walls formed in the embryo. Methylesterified pectins were found both in the intine of non-induced vacuolated microspores and in the newly formed cell walls of the embryo whilst unesterified pectins were not seen in these young walls. JIM4 and JIM8 monoclonal antibodies were used to study arabinogalactan proteins. The AGP epitope located by JIM4 was not present in vacuolated microspores before induction but it did appear after the induction of androgenesis. Epitopes recognised by JIM8 were present in microspores before induction but not in induced microspores. These epitopes reappeared in embryogenic multicellular pollen grains. These results seem to confirm the role of this type of AGP as a signal molecule in the process of androgenesis.
URIhttp://hdl.handle.net/10261/99902
ISSN1335-6372
Appears in Collections:(EEAD) Artículos
(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.