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Bioproduction of 4-vinylphenol from corn cob alkaline hydrolyzate in two-phase extractive fermentation using free or immobilized recombinant E. coli expressing pad gene

AuthorsSalgado, José M.; Rodríguez-Solana, R.; Curiel, José Antonio ; Rivas, Blanca de las ; Muñoz, Rosario ; Domínguez, José Manuel
KeywordsCorn cobs
Extractive fermentation
Immobilized cells
Issue Date10-May-2014
CitationEnzyme and Microbial Technology 58-59: 22-28 (2014)
AbstractIn situ extractive fermentation was used to produce 4-vinyl derivatives from hydroxycinnamic acids extracted from corn cobs by recombinant Escherichia coli cells expressing Lactobacillus plantarum phenolic acid descarboxylase (PAD) gene. This microorganism mainly produced 4-vinylphenol (4VP) from p-coumaric acid (p-CA). In the first study , we observed that the concentrations of 4VP are higher than 1g/L which had a negative impact on decarboxylation of p-CA to 4VP by recombinant E. coli cells. Because of this, and in order to improve the downstream process, a two-phase aqueous-organic solvent system was developed. The results of the extractive fermentation indicated that it was possible to use hydrolyzates as aqueous phase to bioproduce 4VP, and recover simultaneously the product in the organic phase containing hexane. The detoxification of pre-treated corn cob alkaline hydrolyzate improved 4VP production up to 1003.5mg/L after 24h fermentation (QP=41.813mg/Lh). Additionally, preliminary experiments using cells immobilized in calcium alginate showed to be a good system for the biotransform of p-CA to 4VP in extractive fermentation, although the process hindered partially the recovery of 4VP in the organic phase. © 2014 Elsevier Inc.
Publisher version (URL)http://dx.doi.org/10.1016/j.enzmictec.2014.02.005
Identifiersissn: 1879-0909
Appears in Collections:(ICTAN) Artículos
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