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Efficient production of androgenic doubled-haploid mutants in barley by the application of sodium azide to anther and microspore cultures

AuthorsCastillo Alonso, Ana María ; Cistué Sola, Luis ; Vallés Brau, María Pilar ; Sanz Madoz, José Miguel ; Romagosa, Ignacio; Molina-Cano, José Luis
Sodium azide
Doubled haploid
Issue DateFeb-2001
CitationPlant Cell Reports 20 (2): 105-111 (2001)
AbstractThe aim of this study was to establish a protocol for an efficient production of agronomical and/or physiological mutants from model (cvs. Igri and Cobra) and low-androgenic-responding (cv. Volga) cultivars of barley through the application of a mutagenic agent, sodium azide, to anthers and isolated microspores cultured in vitro. This technology offers the possibilities of screening for recessive mutants in the first generation, selecting for novel genotypes from very large haploid populations, avoiding chimerism and rapidly fixing selected genotypes as fertile true breeding lines. The mutagenic treatment, 10-3-10-5 M sodium azide, was applied during the anther induction pre-treatment or immediately after the microspore isolation procedure. Out of 616 M2 doubled-haploid lines characterised under field conditions, a total of 63 morphological and developmental independent mutant lines were identified. The percentage of M2 doubled-haploid lines carrying mutations per line analysed was 3.8% when 10-4 M sodium azide was applied to anthers from the low-responding cv. Volga; this increased to 8.6% and 15.6% when 10-5 and 10-4 M sodium azide were applied to freshly isolated microspores from model cultivars.
Publisher version (URL)http://dx.doi.org/10.1007/s002990000289
Identifiersdoi: 10.1007/s002990000289
issn: 0721-7714
Appears in Collections:(EEAD) Artículos
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