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Capillary electrophoresis-mass spectrometry for peptide analysis: Target-based approaches and proteomics/ peptidomics strategies

AuthorsSimó, Carolina ; Cifuentes, Alejandro ; Kašička, Václav
Target analysis
Issue Date2013
PublisherHumana Press
CitationCapillary Electrophoresis of Biomolecules (Cap.11): 139-151 (2013)
SeriesMethods in Molecular Biology 984
AbstractIn this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgunlike methodology used for proteomic analysis. Particularly, CE-MS using a time-of- flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics. © Springer Science+Business Media, LLC 2013.
Identifiersdoi: 10.1007/978-1-62703-296-4_11
isbn: 978-1-62703-295-7
Appears in Collections:(CIAL) Libros y partes de libros
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