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Global characterization and target identification of piRNAs and endo-siRNAs in mouse gametes and zygotes

AuthorsGarcía-López, Jesús; Hourcade, Juan D.; Alonso, Lola; Cárdenas, David B.; Del Mazo, Jesús
Deep sequencing
Transposable elements
Germ cells
Issue DateJun-2014
CitationBiochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms 2014,1839 ( 6) 463–475
AbstractA set of small RNAs known as rasRNAs (repeat-associated small RNAs) have been related to the down-regulation of Transposable Elements (TEs) to safeguard genome integrity. Two key members of the rasRNAs group are piRNAs and endo-siRNAs. We have performed a comparative analysis of piRNAs and endo-siRNAs present in mouse oocytes, spermatozoa and zygotes, identified by deep sequencing and bioinformatic analysis. The detection of piRNAs and endo-siRNAs in the spermatozoa and revealed also in zygotes, hints to their potential delivery to oocytes during fertilization. However, a comparative assessment of the three cell types indicates that both piRNAs and endo-siRNAs are mainly maternally inherited. Finally, we have assessed the role of the different rasRNA molecules in connection with amplification processes by way of the "ping-pong cycle". Our results suggest that the ping-pong cycle can act on other rasRNAs, such as tRNA- and rRNA-derived fragments, thus not only being restricted to TEs during gametogenesis. © 2014 Elsevier B.V.
Description44 p.-1 tab.-10 fig.
Publisher version (URL)http://dx.doi.org/10.1016/j.bbagrm.2014.04.006
Appears in Collections:(CIB) Artículos
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