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Improvement of a gas chromatographic method for the analysis of iminosugars and other bioactive carbohydrates

AuthorsRodríguez-Sánchez, Sonia ; Soria, Ana C. ; Ruiz-Matute, Ana I. ; Sanz, M. Luz
KeywordsIminosugars GC–MS (SIM)
Injection port temperature
Issue Date2013
CitationJournal of Chromatography A 1289: 145- 148 (2013)
AbstractThe analysis of derivatised iminosugars and other bioactive low molecular weight carbohydrates present at low concentrations in different vegetable extracts (hyacinth, mulberry and buckwheat) required the improvement of a previously developed gas chromatographic method. Among the different parameters optimized, the temperature of the injection port, evaluated for the first time, resulted to be the most important. Thus, 240°C was chosen as a tradeoff to achieve the required volatility, to avoid degradation and to provide symmetric peaks for bioactive carbohydrates highly retained such as glycosyl inositols. GC-MS operating under selective ion monitoring (SIM) acquisition mode and GC-FID provided limits of detection and quantitation for the target compounds of 0.2 and 0.7ngg-1 and of 1 and 3ngg-1 on average, respectively. Therefore, both methodologies could be considered appropriate to extend the range of quantitation of these bioactives. © 2013 Elsevier B.V.
Identifiersdoi: 10.1016/j.chroma.2013.03.013
issn: 0021-9673
e-issn: 1873-3778
Appears in Collections:(IQOG) Artículos
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