English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/99388
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 11 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

Interaction of differently functionalized fluorescent silica nanoparticles with neural stem- and tissue-type cells

Autor Izak-Nau, Emilia; Voetz, Matthias; Eiden, Stefanie; Puntes, Víctor F.
Palabras clave Neural cell types
Silica NP
Toxicity, Uptake
Fluorescence spectrum analysis
Fecha de publicación 2014
EditorTaylor & Francis
Citación Nanotoxicology 8(Supl.1): 138-148 (2014)
ResumenEngineered amorphous silica nanoparticles (SiO2 NPs), due to simple and low cost production, are increasingly used in commercial products and produced on an industrial scale. Despite the potential benefits, there is a concern that exposure to certain types of SiO2 NPs may lead to adverse health effects. As some NPs can cross the blood--brain barrier and may, in addition, reach the central nervous system through the nasal epithelium, this study addresses the responses of different neural tissue-type cells including neural stem cells, neurons, astrocytes and microglia cells to increasing doses of 50 nm fluorescent core/shell SiO2 NPs with different [–NH2, –SH and polyvinylpyrrolidone (PVP)] surface chemistry. The SiO2 NPs are characterized using a variety of physicochemical methods. Assays of cytotoxicity and cellular metabolism indicates that SiO2 NPs cause cell death only at high particle doses, except PVP-coated SiO2 NPs which do not harm cells even at very high concentrations. All SiO2 NPs, except those coated with PVP, form large agglomerates in physiological solutions and adsorb a variety of proteins. Except PVP-NPs, all SiO2 NPs adhere strongly to cell surfaces, but internalization differs depending on neural cell type. Neural stem cells and astrocytes internalize plain SiO2, SiO2–NH2 and SiO2–SH NPs, while neurons do not take up any NPs. The data indicates that the PVP coat, by lowering the particle–biomolecular component interactions, reduces the biological effects of SiO2 NPs on the investigated neural cells.
URI http://hdl.handle.net/10261/99388
Identificadoresdoi: 10.3109/17435390.2013.864427
issn: 1743-5390
e-issn: 1743-5404
Aparece en las colecciones: (CIN2) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.