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Título

Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samples

Autor Juan-Franco, Elena de ; Rodríguez Frade, José M.; Mellado, Mario; Lechuga, Laura M.
Palabras clave hGH
Immunosensor
Isoforms
Serum
Site-directed immobilization
SPR
Fecha de publicación 2013
EditorElsevier
Citación Talanta 114: 268-275 (2013)
ResumenWe have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-antibody sensor surface specific for both hormone isoforms was assembled by using the biotin-streptavidin system. The immunosensor functionality was checked for the direct detection of the 22K hGH isoform in buffer, which gave high specificity and reproducibility (intra and inter-assay mean coefficients of variation of 8.23% and 9% respectively). The selective determination of the 22K and 20K hGH isoforms in human serum samples in a single assay was possible by using two specific anti-hGH monoclonal antibodies. The detection limit for both hormone isoforms was 0.9 ng mL -1 and the mean coefficient of variation was below 7.2%. The excellent reproducibility and sensitivity obtained indicate the high performance of this immunosensor for implementing an anti-doping test. © 2013 Elsevier B.V. All rights reserved.
URI http://hdl.handle.net/10261/99379
DOI10.1016/j.talanta.2013.04.042
Identificadoresdoi: 10.1016/j.talanta.2013.04.042
issn: 0039-9140
e-issn: 1873-3573
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