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Título

Strategy for Modular Tagged High-Throughput Amplicon Sequencing Evaluation of Subsampling-Based Normalization Strategies for Tagged High-Throughput Sequencing Data Sets from Gut Microbiomes

AutorAguirre de Cárcer, Daniel; Denman, Stuart E.; McSweeney, Christopher; Morrison, Mark
Fecha de publicación2-jul-2014
ResumenSeveral subsampling-based normalization strategies were applied to different high-throughput sequencing data sets originating from human and murine gut environments. Their effects on the data sets’ characteristics and normalization efficiencies, as measured by several -diversity metrics, were compared. For both data sets, subsampling to the median rather than the minimum number appeared to improve the analysis.
URIhttp://hdl.handle.net/10261/99319
DOI10.1128/AEM.05491-11
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